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HomeLab Manual/PracticalsB.Pharm 1st SemesterModified Limit Test for Chloride

Modified Limit Test for Chloride

MODIFIED LIMIT TEST FOR CHLORIDES

AIM:

To perform the modified limit test for chlorides for a given sample

APPARATUS:

Nessler cylinder, pipette, glass rod, measuring cylinder.

CHEMICALS REQUIRED:

NaCl, dilute nitric acid, Distilled water, Silver nitrate, Ethanol, Potassium sulphate, Barium sulphate, and Potassium permanganate.

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PRINCIPLE:

Potassium permanganate gives pink color in an aqueous solution which interferes with the general limit test. To decolorize potassium permanganate it is reduced by heating with ethanol, and the precipitate formed is removed by filtration. The colorless filtrate obtained is subjected to the limit test.

PROCEDURE:

1mg of potassium permanganate is heated with 4 ml of ethanol until the color becomes colorless. Filter the solutions for comparison with standards, use 20ml of filtrate, and complete the limit test.

Take two Nessler’s Cylinders, wash them thoroughly, and then label them as STANDARD and TEST

Preparation of Standard chloride solution:

Accurately weigh 0.05845g of NaCl and dissolve in 100 ml of distilled water. (0.05845% w/v of NaCl).

Preparation of standard Solution:

Take 1 ml of NaCl solution and transfer into Nessler’s cylinder and add 10 ml dilute Nitric Acid and make up the volume up to 50 ml with distilled water then add 1 ml of silver nitrate solution. Stir well with a glass rod and keep it aside for 5 min.

Preparation of Test Solution:

Take 20 ml of the above-prepared samples and transfer them into Nessler’s cylinder add 10 ml of dilute Nitric Acid and make up the volume up to 50 ml with distilled water then add 1 ml of silver nitrate solution. Stir well with a glass rod and keep it aside for 5 min.

STANDARD SOLUTION

In a Nessler’s cylinder Chloride std. solution (25ppm Cl) 10ml taken. Add 5 ml water + 10 ml of dil.HNO3.
Dilute to 50 ml in Nessler’s cylinder + 1 ml of 0.1 M AgNO3 solution.
Stir & allow to stand for 5 min.
Observe the opalescence Observation Inference

The opalescence produced in the sample solution is greater than / less than that of the standard solution.

The sample passes/ doesn’t pass the limit test for chlorides.

TEST SOLUTION

20 ml of test solution + 10 ml of dil.HNO3.
Dilute to 50 ml in Nessler’s cylinder + 1 ml of 0.1 M AgNO3 solution.
Stir & allow to stand for 5 min.
Observe the opalescence

The opalescence produced in the test solution is compared with that produced in the standard solution.

OBSERVATION:

REPORT:

The sample________limit test for chlorides.

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