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HomeB.Pharm1st SemesterLimit test for Chloride practical

Limit test for Chloride practical

Limit Test of Chloride

 

Principle:

Limit test of chloride is based on the reaction of soluble chloride with silver nitrate in presence of dilute nitric acid to form silver chloride, which appears as solid particles (Opalescence) in the solution.

Procedure:

Test sampleStandard compound
Specific weight of compound is dissolved in water or solution is prepared as directed in the pharmacopoeia and transferred in Nessler cylinderTake 1ml of 0.05845 % W/V solution of sodium chloride in Nessler cylinder
Add 1ml of nitric acidAdd 1ml of nitric acid
Dilute to 50ml in Nessler cylinderDilute to 50ml in Nessler cylinder
Add 1ml of AgNO3 solutionAdd 1ml of AgNO3 solution
Keep aside for 5 minKeep aside for 5 min
Observe the Opalescence/TurbidityObserve the Opalescence/Turbidity

Observation:

The opalescence produce in sample solution should not be greater than standard solution. If opalescence produces in sample solution is less than the standard solution, the sample will pass the limit test of chloride and visa versa.

Reasons:

Nitric acid is added in the limit test of chloride to make solution acidic and helps silver chloride precipitate to make solution turbid at the end of process.

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